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Title

Yeast cultures with UCP1 uncoupling activity as a heating device

AuthorsDelás, Joaquina; Notari, Meritxell; Forés, Jaume; Pechuan, Joaquín; Porcar, Manuel; Navarro, Emilio; Montagud, Arnau; Baguena, Minerva; Peretó, Juli; Córdoba, Pedro ; González-Barroso, M. Mar ; Rial, Eduardo ; Moya-Simarro, Andrés; Urchueguía, Javier
KeywordsCalorimetry
instrumentation
ion Channels
metabolism
mitochondrial Proteins
Saccharomyces cerevisiae
cytology
culture
heating
devices
Issue Date31-Dec-2009
PublisherElsevier
CitationNew Biotechnology 26(6):300-306(2009)
AbstractUncoupling proteins (UCPs) are mitochondrial transporters that facilitate controlled dissipation of the proton gradient and thus regulate energetic efficiency. The heat generating capacity of UCP from brown adipose tissue was investigated in yeasts expressing the protein recombinantly under conditions in which the temperature of the growth medium was measured directly. A Liquid Culture Calorimeter (LCC) was built consisting of a thermally isolated culture flask able to keep yeast cultures warm without resorting to additional heating. The exact internal temperature of the cultures was monitored for 24 h through a thermocouple connected to a data logger. Under these conditions, significant temperature increases (1 °C) in the media were recorded when yeast strains expressing endogenously active UCP1 mutants were grown. This is the first direct evidence, in a eukaryotic microbial model, of a temperature rise associated with uncoupling activity, and could be seen as the first step toward developing a biological heating device
Description7 páginas, 5 figuras, 3 tablas -- PAGS nros. 300-306
Publisher version (URL)http://dx.doi.org/10.1016/j.nbt.2009.09.005
URIhttp://hdl.handle.net/10261/66180
DOI10.1016/j.nbt.2009.09.005
ISSN1871-6784
Appears in Collections:(CIB) Artículos
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