Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/65878
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Developmental Expression of Kv Potassium Channels at the Axon Initial Segment of Cultured Hippocampal Neurons |
Autor: | Sánchez-Ponce, Diana CSIC; DeFelipe, Javier CSIC ORCID ; Garrido Jurado, Juan José CSIC ORCID ; Muñoz Céspedes, Alberto CSIC ORCID | Fecha de publicación: | 2012 | Editor: | Public Library of Science | Citación: | PLoS ONE (2012), 7(10): e48557 | Resumen: | Axonal outgrowth and the formation of the axon initial segment (AIS) are early events in the acquisition of neuronal polarity. The AIS is characterized by a high concentration of voltage-dependent sodium and potassium channels. However, the specific ion channel subunits present and their precise localization in this axonal subdomain vary both during development and among the types of neurons, probably determining their firing characteristics in response to stimulation. Here, we characterize the developmental expression of different subfamilies of voltage-gated potassium channels in the AISs of cultured mouse hippocampal neurons, including subunits Kv1.2, Kv2.2 and Kv7.2. In contrast to the early appearance of voltage-gated sodium channels and the Kv7.2 subunit at the AIS, Kv1.2 and Kv2.2 subunits were tethered at the AIS only after 10 days in vitro. Interestingly, we observed different patterns of Kv1.2 and Kv2.2 subunit expression, with each confined to distinct neuronal populations. The accumulation of Kv1.2 and Kv2.2 subunits at the AIS was dependent on ankyrin G tethering, it was not affected by disruption of the actin cytoskeleton and it was resistant to detergent extraction, as described previously for other AIS proteins. This distribution of potassium channels in the AIS further emphasizes the heterogeneity of this structure in different neuronal populations, as proposed previously, and suggests corresponding differences in action potential regulation. | URI: | http://hdl.handle.net/10261/65878 | DOI: | 10.1371/journal.pone.0048557 | Identificadores: | doi: 10.1371/journal.pone.0048557 issn: 1932-6203 |
Aparece en las colecciones: | (IC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
DeFelipe,2012,PlosOne.pdf | 5,2 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
15
checked on 22-abr-2024
SCOPUSTM
Citations
26
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
25
checked on 28-feb-2024
Page view(s)
302
checked on 22-abr-2024
Download(s)
371
checked on 22-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.