English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/65876
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Enhanced cAMP response element-binding protein activity increases neuronal excitability, hippocampal long-term potentiation, and classical eyeblink conditioning in alert behaving mice

AuthorsGruart, Agnès; Benito, Eva; Delgado-García, José María; Barco, Ángel
Issue Date2012
PublisherSociety for Neuroscience
CitationJournal of Neuroscience 32(48): 17431-17441 (2012)
AbstractThe activity-regulated transcription factor cAMP response element-binding protein (CREB) is an essential component of the molecular switch that controls the conversion of short-term into long-term forms of plasticity, including those underlying long-term memory. Previous research in acute brain slices of transgenic animals expressing constitutively active CREB variants has revealed that enhancing CREB activity increases the intrinsic excitability of neurons and facilitates the late phase of long-term potentiation (LTP) in the Schaffer collateral pathway. Here, we report similar changes in plasticity at the Schaffer collateral pathway in alert behaving mice. Forebrain expression of a strong constitutively active CREB variant, VP16-CREB, enhanced in vivo LTP evoked in the Schaffer collateral pathway and caused significant changes in the input/output curve andpaired-pulse facilitationinCA3-CA1 synapses,which could be explained by the increased excitability ofhippocampal pyramidal neurons.In addition, classical eyeblink conditioning intransgenic mice and control littermates showed larger conditioned responses in mutant mice that were associated to at ransient increase in the acquisition rate and in the concomitant learning-dependent change in synaptic strength. The sustained chronic activation of CREB activity, however, impaired the performance in this task. Our experiments demonstrate that the sustained enhancement of CREB function alters the physiology and plasticity of hippocampal circuits in behaving animals and that these changes have important consequences in associative learning. © 2012 the authors.
Identifiersdoi: 10.1523/JNEUROSCI.4339-12.2012
issn: 0270-6474
e-issn: 1529-2401
Appears in Collections:(IN) Artículos
Files in This Item:
File Description SizeFormat 
Enhanced cAMP Response Element.pdf3,4 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.