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Title

Cell wall composition and protoplast regeneration in Candida albicans

AuthorsElorza, M. Victoria
Issue Date1983
PublisherSpringer
CitationAntonie van Leeuwenhoek 49(4-5): 457-469 (1983)
AbstractThe transition of blastospores to the mycelial phase in Candida albicans was induced after the blastospores were kept at 4°C for several hours and then transferred to a fresh medium prewarmed at 37°C. Glucan was the most abundant polymer in the wall in the two morphogenetic forms but the amount of chitin was higher in the mycelial form than in blastospores. Efficient protoplasting required reducing agents and proteases together with β-glucanases (zymolyase). Protein synthesis in regenerating protoplasts was initiated after about 30 min. Chitin synthetase, initially very low, was incorporated in important amounts into cell membranes mainly in a zymogenic state. After a few hours chitin was the most abundant polymer found in the aberrant wall of the regenerating protoplast.
URIhttp://hdl.handle.net/10261/65812
DOI10.1007/BF00399324
Identifiersdoi: 10.1007/BF00399324
issn: 0003-6072
e-issn: 1572-9699
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