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dc.contributor.authorMartínez, Virginia-
dc.contributor.authorJurkevitch, Edouard-
dc.contributor.authorGarcía, José Luis-
dc.contributor.authorPrieto, María Auxiliadora-
dc.date.accessioned2013-02-04T09:02:49Z-
dc.date.available2013-02-04T09:02:49Z-
dc.date.issued2012-12-
dc.identifier.citationEnvironmental Microbiology, (2012)es_ES
dc.identifier.issn1462-2912-
dc.identifier.urihttp://hdl.handle.net/10261/65747-
dc.description12 páginas, 4 figuras, 3 tablases_ES
dc.description.abstractBdellovibrio bacteriovorus HD100 is an obligate predator that invades and grows within the periplasm of Gram-negative bacteria, including mcl-polyhydroxyalkanoate (PHA) producers such as Pseudomonas putida. We investigated the impact of prey PHA content on the predator fitness and the potential advantages for preying on a PHA producer. Using a new procedure to control P. putida KT2442 cell size we demonstrated that the number of Bdellovibrio progeny depends on the prey biomass and not on the viable prey cell number or PHA content. The presence of mcl-PHA hydrolysed products in the culture supernatant after predation on P. putida KT42Z, a PHA producing strain lacking PhaZ depolymerase, confirmed the ability of Bdellovibrio to degrade the prey's PHA. Predator motility was higher when growing on PHA accumulating prey. External addition of PHA polymer (latex suspension) to Bdellovibrio preying on the PHA minus mutant P. putida KT42C1 restored predator movement, suggesting that PHA is a key prey component to sustain predator swimming speed. High velocities observed in Bdellovibrio preying on the PHA producing strain were correlated to high intracellular ATP levels of the predator. These effects brought Bdellovibrio fitness benefits as predation on PHA producers was more efficient than predation on non-producing bacteriaes_ES
dc.description.sponsorshipThis work was supported by the Ministry of Economía y Competitividad (BIO2010-21049, CSD2007-00005)es_ES
dc.language.isoenges_ES
dc.publisherBlackwell Publishinges_ES
dc.rightsopenAccesses_ES
dc.titleReward for Bdellovibrio bacteriovorus for preying on a polyhydroxyalkanoate produceres_ES
dc.typeartículoes_ES
dc.identifier.doi10.1111/1462-2920.12047-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1111/1462-2920.12047es_ES
dc.identifier.e-issn1462-2920-
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