English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/65662
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

A genomic approach for the identification and classification of genes involved in cell wall formation and its regulation in Saccharomyces cerevisiae

AutorGroot, Piet W. J. de; Vázquez de Aldana, Carlos R.; Dueñas, Encarnación; Rey Iglesias, Francisco del; Pérez González, Pilar; García, Juan C.; García, Luis J.
Fecha de publicación2001
EditorHindawi Publishing Corporation
John Wiley & Sons
CitaciónComparative and Functional Genomics 2(3): 124-142 (2001)
ResumenUsing a hierarchical approach, 620 non-essential single-gene yeast deletants generated by EUROFAN I were systematically screened for cell-wall-related phenotypes. By analyzing for altered sensitivity to the presence of Calcofluor white or SDS in the growth medium, altered sensitivity to sonication, or abnormal morphology, 145 (23%) mutants showing at least one cell wall-related phenotype were selected. These were screened further to identify genes potentially involved in either the biosynthesis, remodeling or coupling of cell wall macromolecules or genes involved in the overall regulation of cell wall construction and to eliminate those genes with a more general, pleiotropic effect. Ninety percent of the mutants selected from the primary tests showed additional cell wall-related phenotypes. When extrapolated to the entire yeast genome, these data indicate that over 1200 genes may directly or indirectly affect cell wall formation and its regulation. Twenty-one mutants with altered levels of β1,3-glucan synthase activity and five Calcofluor white-resistant mutants with altered levels of chitin synthase activities were found, indicating that the corresponding genes affect β1,3-glucan or chitin synthesis. By selecting for increased levels of specific cell wall components in the growth medium, we identified 13 genes that are possibly implicated in different steps of cell wall assembly. Furthermore, 14 mutants showed a constitutive activation of the cell wall integrity pathway, suggesting that they participate in the modulation of the pathway either directly acting as signaling components or by triggering the Slt2-dependent compensatory mechanism. In conclusion, our screening approach represents a comprehensive functional analysis on a genomic scale of gene products involved in various aspects of fungal cell wall formation.
DescripciónThis is an open access article distributed under the Creative Commons Attribution License.-- et al.
URIhttp://hdl.handle.net/10261/65662
DOI10.1002/cfg.85
Identificadoresdoi: 10.1002/cfg.85
issn: 1531-6912
e-issn: 1532-6268
Aparece en las colecciones: (IMB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
A Genomic Approach.pdf251,16 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.