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Title

20S RNA narnavirus defies the antiviral activity of SKI1/XRN1 in Saccharomyces cerevisiae

AuthorsEsteban, Rosa ; Vega Hernández, Lorena; Fujimura, Tsutomu
Issue Date2008
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry 283(38): 25812-25820 (2008)
Abstract20S RNA virus is a persistent positive strand RNA virus found in Saccharomyces cerevisiae. We previously observed that the virus generated in vivo from a launching vector possessed the correct RNA termini without extra sequences. Here we present evidence that the SKI1/XRN1 5′-exonuclease plays a major role in the elimination of the non-viral upstream sequences from the primary transcripts. The virus, once generated, however, is fairly unaffected by overexpression or deletion of SKI1/XRN1. By contrast, the copy number of the L-A double-stranded RNA virus in the same host is greatly increased by the deletion of SKI1/XRN1, and overexpression of the gene cured L-A virus from the cells at a high frequency. 20S RNA virus, unlike L-A virus, has a strong secondary structure at its 5′-end: the first four nucleotides are G, and they are buried at the bottom of a long stem structure, features known to inhibit the SKI1/XRN1 5′-exonuclease progression. Mutations that weakened the 5′-stem structure made 20S RNA virus vulnerable to SKI1/XRN1. These results, together with the data on L-A virus, indicate a strong anti-RNA virus activity of SKI1/XRN1. Given that 20S RNA virus resides and replicates in the cytoplasm without a protective capsid, our results suggest that the strong secondary structure at the 5′-end is crucial for the 20S RNA virus to evade the host SKI1/XRN1 defense. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.
DescriptionEl pdf del artículo es la versión post-print.
Publisher version (URL)http://dx.doi.org/10.1074/jbc.M804400200
URIhttp://hdl.handle.net/10261/65621
DOI10.1074/jbc.M804400200
Identifiersdoi: 10.1074/jbc.M804400200
issn: 0021-9258
e-issn: 1083-351X
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