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Nucleotide Sequence and Deduced Functions of a Set of Cotranscribed Genes of Streptomyces coelicolor A3(2) Including the Polyketide Synthase for the Antibiotic Actinorhodin*

AuthorsFernández-Moreno, Miguel Ángel ; Martínez, Eduardo; Boto, Luis ; Malpartida Romero, Francisco
Issue Date25-Sep-1992
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry, 267(27):19278-19290 (1992)
AbstractA 5.3-kb region of the Streptomyces coelicolor actinorhodin gene cluster, including the genes for polyketide biosynthesis, was sequenced. Six identified open reading frames (ORF1-6) were related to genetically characterized mutations of classes actI, VII, IV, and VB by complementation analysis. ORF1-6 run divergently from the adjacent act111 gene, which encodes the polyketide synthase (PKS) ketoreductase, and appear to form an operon. The deduced gene products of ORF1-3 are similar to fatty acid synthases (FAS) of different organisms and PKS genes from other polyketide producers. The predicted ORF5 gene product is similar to type I1 &lactamases of Bacillus cereus and Bacteroides fragilis. The ORF6 product does not resemble other known proteins. combining the genetical, biochemical, and similarity data, the potential activities of the products of the six genes can be postulated as: 1) condensing enzyme/acyl transferase (ORF1 + ORF2); 2) acyl carrier protein (ORF3); 3) putative cyclase/dehydrase (ORF4); 4) dehydrase (ORF5); and 5) “dimerase” (ORF6). The data show that the actinorhodin PKS consists of discrete monofunctional components, like that of the Escherichia coli (Type 11) FAS, rather than the multifunctional polypeptides for the macrolide PKSs and vertebrate FASs (Type I).
Description13 páginas, 6 figuras, 3 tables et al
Publisher version (URL)http://www.jbc.org/content/267/27/19278.full.pdf
Appears in Collections:(MNCN) Artículos
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