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A retrotransposon sequence is related to DNA instability in barley microspore culture

AuthorsMuñoz-Amatriaín, María ; Cistué Sola, Luis ; Castillo Alonso, Ana María ; Romagosa, Ignacio; Vallés Brau, María Pilar
Doubled haploid
Genetic stability
Microspore culture
Issue DateSep-2004
PublisherEucarpia (European Association for Research on Plant Breeding)
Citation17th Eucarpia General Congress: Genetic variation for plant breeding (Tullin, Austria. 8 - 11 September 2004)
AbstractIn order to assay gametoclonal variation in barley, a set of double haploid (DH) plants (cv. Igri) regenerated from isolated microspore culture by direct embryogenesis, was especially developed. AFLP analysis was performed with eleven enzyme-primer combination, that represented the study of 719 band polymorphisms. Only one change in the band patterns was observed, indicating a high degree of genetic stability. An extra band was present in DH lines regenerated from microspores, treated or not with sodium azide, representing a “hot spot” of instability. The sequence of this band was related to barley “sukkula” retrotransposon element. Our preliminary results suggest the activation of retrotransposon by barley isolated microspore culture.
Description3 Pags., 1 Fig.
Appears in Collections:(EEAD) Comunicaciones congresos
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