English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/65441
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Effect of nitrate on oeroxisome ultrastructure and catalase activity in nodules of Lupinus albus L. cv. Multolupa

AuthorsLorenzo, Cristina de; Lucas, M. Mercedes ; Vivo Rodríguez, A.; Felipe, Mª Rosario de
KeywordsPeroxisomes
Diamino-benzidine
Nitrate
Catalese
Leghaemoglobin
Issue Date1990
PublisherSociety for Experimental Biology and Medicine
CitationJounal of Experimental Botany 41(233):1573.1578(1990)
AbstractThis paper reports on an ultrastructural study of peroxisomes in infected nodule cells of Lupinus albus L. cv. Multolupa plants grown with and without nitrate (in short-term experiments). Cytochemical localizaron of catalasc and peroxidase was performed applying the diamino-benzidine (DAB) technique in these tissues. The infected cclls presented a mean of seven peroxisomes, the máximum being 16 in some cells. Peroxisome shapes proved to be fairly round or egg-shaped, with máximum and minimum diameter means of 0-35 and 0-18 ^m, respectively. They were preferentially positioned on the cell periphery. The intense osmiophilic staining obtained by applying the DAB technique indícales a strong catalase activity reaction in these microbodies. The addition of nitrate (20 mol m~3 ) to the growing plants exerted a negativo effcct on nitrogenase activity, which diminished by 31-6% after 5d of treatment. Severe alterations in the ultrastructure of microbodies, bacteroids, and peribacteroidal membranes were observed. Cytochemical data show a reduction in catalase localization in peroxisomes and an increased peroxidase activity in the cytosol. Finally, leghaemoglobin (Lb) localization was studied in nitrate-grown plants, confirming our previous observation of a decrease in this protein. Discussion focuses on the involvement of these results in tissue senescence of the nodules following nitrate application
Description6 páginas, ilustraciones y tablas estadísticas.
URIhttp://hdl.handle.net/10261/65441
ISSN0022.0957
Appears in Collections:(ICA) Artículos
Files in This Item:
File Description SizeFormat 
jounexpbotany1990086.pdf4,59 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.