English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/65057
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Adherence kinetics, resistance to benzalkonium chloride and microscopic analysis of mixed biofilms formed by Listeria monocytogenes and Pseudomonas putida

AuthorsSaá Ibusquiza, Paula ; Rodríguez Herrera, Juan José ; Vázquez-Sánchez, Daniel ; López Cabo, Marta
KeywordsBiofilm
Disinfection
Listeria monocytogenes
Pseudomonas putida
Benzalkonium chloride
Electronic microscopy
Issue Date2012
PublisherElsevier
CitationFood Control 25(1) 202-210 (2012)
AbstractComparison between the resistance to BAC and the microscopic structure between mixed-species biofilms formed by different strains of Listeria monocytogenes and Pseudomonas putida CECT 845 under different scenarios and that obtained by the corresponding monospecies L. monocytogenes biofilm was carried out. The association of P. putida with L. monocytogenes quickens biofilm formation and increases significantly (p < 0.05) the BAC-resistance of the biofilm after 4 days of incubation at 25 °C respecting to that formed by monospecies biofilms. According with the adherence profiles of P. putida, two different patterns of association between both species (A and B) were identified, being type A pattern found in the mixed biofilms much more resistant to BAC. After 11 days of incubation, a destructuration of mixed biofilms occurred in all experimental assays, being in 2 out of 5 experimental cases (4032 and BAC-adapted 5873 on polypropylene) accompanied by a sharp decrease in the number of adhered cells. Microscopic analyses demonstrated that complex three-dimensional microscopic structure showed the highest resistance to BAC (4032-SS). Obtained results clearly highlight that to improve disinfection protocols for assuring food safety, it is necessary to mimick those bacterial association that occur in nature
Description9 páginas, 6 figuras, 1 tabla
Publisher version (URL)http://dx.doi.org/10.1016/j.foodcont.2011.10.002
URIhttp://hdl.handle.net/10261/65057
DOI10.1016/j.foodcont.2011.10.002
ISSN0956-7135
Appears in Collections:(IIM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.