English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/65023
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Post-transcriptional control of a glucoamylase gene from Trichoderma harzianum under stress conditions

AuthorsDana, María de la Mercedes; Pintor-Toro, José Antonio
Issue Date2005
PublisherBlackwell Publishing
CitationMolecular Microbiology 57(1): 250-260 (2005)
AbstractWe have examined the regulation of Trichoderma harzianum glucoamylase gene (Gla66) in response to different growth conditions. Transcription of the Gla66 gene is initiated from two different sites, yielding two transcripts of 2.1 kb and 2.6 kb respectively. The 2.1 kb mRNA (ST) encodes for an extracellular glucoamylase of 66 kDa. This protein shows the domains conserved in other fungal glucoamylases: a signal peptide responsible for protein secretion and a catalytic domain, both joined by a linker region. The longest transcript (LT) is untranslated, it contains an unusually extended 5′-untranslated region and is transcribed under stress and growth limiting conditions. The translational control of LT could be defined by the presence of four upstream open reading frames (uORFs) in its 5′-leader sequence. The analysis of these uORFs in a yeast heterologous system shows that two of these uORFs prevent the Gla66 translation under unfavourable growth conditions, when the LT transcript is accumulated.
Identifiersdoi: 10.1111/j.1365-2958.2005.04682.x
issn: 0950-382X
e-issn: 1365-2958
Appears in Collections:(IRNAS) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.