English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/64355
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Localized synthesis of the outer envelope from Thermus thermophilus

AuthorsAcosta, Federico; Álvarez, Laura ; Pedro, Miguel Ángel de ; Berenguer, José
KeywordsThermus
Outer membrane
S-layer
Issue Date2012
PublisherSpringer
CitationExtremophiles 16(2): 267-275 (2012)
AbstractIn agreement with its distinct phylogenetic origin, the envelope of Thermus thermophilus consists of a complex pattern of layers with properties intermediate between those of Gram positives and Proteobacteria. Its cell wall of Gram positive composition is surrounded by an outer envelope that includes a crystalline layer scaffold built up by the SlpA protein, lipids and polysaccharides. The synthesis of this outer envelope has been studied by confocal microscopy. Available amino groups from the cell surface, mainly belonging to the SlpA protein, were covalently labelled in vivo with fluorescent dyes. Stained cells were able to grow without any apparent loss of viability, allowing the localization of the regions of new synthesis as dark nonfluorescent spots. Our results demonstrate that the outer envelope of T. thermophilus is synthesized from a central point in the cells, likely following a helical pattern. Cell poles and subpolar regions are basically inert and retain their label for generations.
Publisher version (URL)http://dx.doi.org/10.1007/s00792-011-0427-7
URIhttp://hdl.handle.net/10261/64355
DOI10.1007/s00792-011-0427-7
ISSN1431-0651
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.