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Title

Histochemical characterisation of oocytes of the swordfish Xiphias gladius

Other TitlesCaracterización histoquímica de ovocitos de pez espada, Xiphias Gladius
AuthorsOrtiz-Delgado, Juan B. CSIC ORCID ; Sarasquete, Carmen CSIC ORCID ; Porcelloni, Serena; Fossi, Cristina
KeywordsHistochemistry
Carbohydrates
Lipids
Glycoconjugates
Vitellogenin
Zona radiata protein
Methallothionein
Thyroid homones
Cations
Oocytes
Xiphias gladius
Histoquímica
Carbohidratos
Lípidos
Glicoconjugados
Vitelogenina
Proteína radiada
Metalotioneinas
Hormonoas tiroideas
Castiones
Ovocitos
Issue Date2008
PublisherCSIC - Instituto de Ciencias del Mar (ICM)
CitationSci. Mar. 72(3): 549-564
Abstract[EN] This paper reports a histological, histochemical and immunohistochemical characterisation of growing oocytes of the swordfish Xiphias gladius. The presence and distribution of carbohydrates, proteins, lipids, calcium, iron, vitellogenin/Vg, zona radiata protein/Zrp, metallothionein/Mt, and thyroid hormones/T3-T4 were studied during oogenesis (cortical alveoli, globules, yolk-granules, cytoplasm, follicular and radiata envelopes). During the initial vitellogenic phase, the oocytes showed cortical alveoli and oil globules containing neutral lipids exclusively. During this phase, small yolk granules appeared around the peripheral cytoplasm, and they increased through exogenous vitellogenesis. Yolk granules were composed of glycoproteins, calcium, iron, and proteins rich in lysine, arginine, tyrosine, tryptophan, cysteine and cystine. Vg and Mt were immunohistochemically detected in yolk. The follicular envelope contained proteins rich in amino acids. Moreover, calcium and thyroid hormones (triiodotyronine and thyroxine/T3, T4) were detected in this cell envelope. Cortical alveoli, which contained carboxylated and neutral glycoconjugates, were especially rich in N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, galactose and sialic acid. Finally, the zona radiata was mainly proteinaceous in nature and was composed of calcium and neutral glycoproteins. The egg envelope or chorion and the liver showed specific immunoreactivities by using anti-salmon Zrp as the primary antiserum
[ES] En este trabajo se realiza una caracterización histológica, histoquímica e inmunohistoquímica de los ovocitos en crecimiento del pez espada, Xiphias gladius. Se estudia la presencia y distribución de carbohidratos, proteínas, lípidos, calcio, hierro, vitelogenina/Vg, proteína radiada/Zrp, metalotioneina/Mt y hormonas tiroideas/ T3-T4 durante la ovogénesis (alveolos corticales, glóbulos, gránulosvitelo, citoplasma, capas folicular y radiada). Durante la fase inicial de la vitelogénesis, los ovocitos presentan alveolos corticales y una gota lipídica conteniendo exclusivamente lípidos neutros. Durante esta fase en la periferia del citoplasma aparecen pequeños granulos de vitelo, incrementando progresivamente durante la vitelogénesis exógena. Los gránulos de vitelo están compuestos por glicoproteínas, calcio, hierro, proteínas ricas en lisina, arginina, tirosina, triptófano, cisteina y cistina, y contienen Vg y Mt. La capa folicular de los ovocitos del pez espada está compuesta por glicoproteínas y proteínas ricas en diferentes aminoácidos. Esta envuelta contiene calcio y hormonas tiroideas/ T3 y T4. Los alveolos corticales están constituidos por glicoconjugados carboxilados y neutros, y son especialmente ricos en N-acetil-D-galactosamina, N-acetil- D-glucosamina, galactosa y ácido siálico. Finalmente, la capa radiada, fundamentalmente proteica, contiene calcio y glicoproteínas neutras. Utilizando anti-salmon Zrp, como anticuerpo primario, el corion o capa radiada y el hígado muestran una immunoreactividad específica.
DescriptionPublicación online disponible en: http://www.icm.csic.es/scimar/index.php
URIhttp://hdl.handle.net/10261/6421
ISSN0214-8358
E-ISSN1886-8134
Appears in Collections:(ICMAN) Artículos

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