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Flow cytometry discrimination between bacteria and clay-humic acid particles during growth-linked biodegradation of phenanthrene by Pseudomonas aeruginosa 19SJ

AuthorsResina Pelfort, O.; García-Junco, M.; Ortega Calvo, J. J. ; Comas Riu, J.; Vives Rego, J.
Issue Date2003
PublisherBlackwell Publishing
CitationFEMS Micriobiology Ecology 43(1): 55-61 (2003)
AbstractMethods that quickly assess microbial density and aggregation in soil and sediments are needed in environmental microbiology. We report a flow cytometry method that uses the green and orange emission of the fluorochrome SYTO-13 to discriminate between bacteria and clay-humic acid particles. This approach distinguishes single or clustered bacteria, and clusters of bacteria and abiotic particles during the growth of the biosurfactant-producing strain Pseudomonas aeruginosa 19SJ on solid phenanthrene in the presence of humic acid-clay complexes.
Identifiersdoi: 10.1016/S0168-6496(02)00399-9
issn: 0168-6496
e-issn: 1574-6941
Appears in Collections:(IRNAS) Artículos
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