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Title

Purification and characterization of the isopenilicin N epimerase from Nocardia lactamdurans

AuthorsLaiz Trobajo, L. ; Liras Padín, P.; Castro González, José Mª; Martín Martín, Juan Fco.
Issue DateApr-1990
PublisherSociety for General Microbiology
CitationJournal of General Microbiology 136: 663-671 (1990)
AbstractIsopenicillin N (IPN) epimerase, an enzyme involved in cephalosporin and cephamycin biosynthesis that converts IPN into penicillin N, was extracted from Nocardia lactamdurans and purified 88-fold. The enzyme was unstable but could be partially stabilized by addition of pyridoxal phosphate. The purified enzyme did not require ATP for activity in contrast to other amino acid racemases. The enzyme had an Mr of 59000 as determined by gel filtration; IPN epimerase from Streptomyces clavuligerus had an Mr of 63000. A protein band of Mr 59000 was found to be enriched in SDS-PAGE of active fractions from N. lactamdurans. The optimal temperature of the epimerase was 25°C and the optimal pH 7.0. The apparent Km for IPN was 270 μM. Fe2+, Cu2+, Hg2+ and Zn2+ strongly inhibited enzyme activity. α-Aminoadipic acid, valine, glutamine, glycine, aspartic acid and glutathione do not affect enzyme activity, whereas ammonium sulphate was inhibitory. The epimerase activity was partially inhibited by several thiol-specific reagents.
Description9 pages, 5 figures, 4 tables, 30 references.
Publisher version (URL)http://dx.doi.org/10.1099/00221287-136-4-663
URIhttp://hdl.handle.net/10261/63775
DOI10.1099/00221287-136-4-663
ISSN1350-0872
E-ISSN1465-2080
Appears in Collections:(IRNAS) Artículos
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