Importance of mesophyll diffusion conductance in estimation of plant photosynthesis in the field

Abstract

Mesophyll diffusion conductance to CO2 (gm) is an important leaf characteristic determining the drawdown of CO2 from substomatal cavities (Ci) to chloroplasts (CC). Finite gm results in modifications in the shape of the net assimilation (A) versus Ci response curves, with the final outcome of reduced maximal carboxylase activity of Rubisco (Vcmax), and a greater ratio of the capacity for photosynthetic electron transport to Vcmax (Jmax/Vcmax) and alterations in mitochondrial respiration rate (Rd) when estimated from A/Ci responses without considering gm. The influence of different Farquhar et al. model parameterizations on daily photosynthesis under non-stressed (Ci kept constant throughout the day) and stressed conditions (mid-day reduction in Ci) was compared. The model was parameterized on the basis of A/CC curves and A/Ci curves using both the conventional fitting procedure (Vcmax and Rd fitted separately to the linear part of the response curve and Jmax to the saturating part) and a procedure that fitted all parameters simultaneously. The analyses demonstrated that A/Ci parameterizations overestimated daily assimilation by 6-8% for high gm values, while they underestimated if by up to 70% for low gm values. Qualitative differences between the A/Ci and A/CC parameterizations were observed under stressed conditions, when underestimated Vcmax and overestimated Rd of A/Ci parameterizations resulted in excessive mid-day depression of photosynthesis. Comparison with measured diurnal assimilation rates in the Mediterranean sclerophyll species Quercus ilex under drought further supported this bias of A/Ci parameterizations. While A/Ci parameterization predicted negative carbon balance at mid-day, actual measurements and simulations with the A/CC approach yielded positive carbon gain under these conditions. In addition, overall variation captured by the best A/Ci parameterization was poor compared with the A/CC approach. This analysis strongly suggests that for correct parameterization of daily time-courses of photosynthesis under realistic field conditions, gm must be included in photosynthesis models.