English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/63435
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Supersensitivity to μ-opioid receptor-mediated inhibition of the adenylyl cyclase pathway involves pertussis toxin-resistant Gα protein subunits

AuthorsMostany, Ricardo; Díaz, Álvaro ; Valdizán, Elsa M. ; Rodríguez-Muñoz, María; Garzón, Javier ; Hurlé, María A.
Issue Date2008
PublisherElsevier
CitationNeuropharmacology 54(6): 989- 997 (2008)
AbstractSustained administration of opioids leads to antinociceptive tolerance, while prolonged association of L-type Ca2+ channel blockers (e.g. nimodipine) with opioids results in increased antinociceptive response. Herein, we investigated the changes in μ-opioid receptor signalling underlying this shift from analgesic tolerance to supersensitivity. Thus, the interaction of μ-opioid receptors with G proteins and adenylyl cyclase was examined in lumbar spinal cord segments of rats. In control animals, the μ-opioid selective agonists, sufentanil and DAMGO, stimulated [35S]5′-(gamma-thio)-triphosphate ([35S]GTPγS) binding and inhibited forskolin-stimulated adenylyl cyclase activity, through a mechanism involving pertussis toxin (PTX) sensitive Gαi/o subunits. Seven days of chronic sufentanil treatment developed antinociceptive tolerance associated with a reduction in μ-agonist-induced [35S]GTPγS binding, μ-agonist-induced adenylyl cyclase inhibition, and co-precipitation of Gαo, Gαi2 Gαz and Gαq11 subunits with μ-opioid receptors. In contrast, combined nimodipine treatment with sufentanil over the same period increased the sufentanil analgesic response. This antinociceptive supersensitivity was accompanied by a significant increase of μ-agonist-induced inhibition of adenylyl cyclase that was resistant to the antagonism by PTX. In good agreement, co-precipitation of the PTX-resistant, Gαz and Gαq/11 subunits with μ-opioid receptors was not lowered. On the other hand, the PTX-sensitive subunits, Gαi2 and Gαo, as well as agonist-stimulated [35S]GTPγS binding were still reduced. Our results demonstrate that μ-opioid analgesic tolerance follows uncoupling of spinal μ-opioid receptors from their G proteins and linked effector pathways. Conversely, the enhanced analgesic response following combined nimodipine treatment with sufentanil is associated with adenylyl cyclase supersensitivity to the opioid inhibitory effect through a mechanism involving PTX-resistant G protein subunits. © 2008 Elsevier Ltd. All rights reserved.
URIhttp://hdl.handle.net/10261/63435
DOI10.1016/j.neuropharm.2008.02.004
Identifiersdoi: 10.1016/j.neuropharm.2008.02.004
issn: 0028-3908
Appears in Collections:(IC) Artículos
(IBBTEC) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.