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Título : Production and characterization of murine monoclonal antibodies against human podocalyxin
Autor : Rodríguez, Ramón B.; Butta, Nora ; Larrucea, Susana ; Alonso, Sonia ; Parrilla, Roberto L.
Palabras clave : Anti-podocalyxin antibodies
Expression pattern
HEK293
K562
Meg01
Tera-1 cells
Fecha de publicación : 7-nov-2006
Editor: Blackwell Publishing
Citación : Tissue Antigens 68(5):407-417(2006)
Resumen: Podocalyxin (podxl) is a protein with a peptide bone of ∼55.5 kDa that undergoes a post-translational glycosylation, yielding a final molecular mass from ∼145 to ∼200 kDa. This protein is normally found covering the vascular side of the epithelial glomerular cells, the podocytes, and its presence is essential to maintain a normal renal function. It has also been reported in other cells and tissues although its function has not been yet clarified. The carboxy-terminal intracellular domain of podxl is nearly 100% identical in most species; however, the ectodomain shows considerable variations although the cysteine residues are conserved. Detection of this protein is elusive, most likely due to differences in post-translational modifications. We aimed at producing murine monoclonal antibodies against human podxl. Immunization with Chinese hamster ovarian -hpodxl-green fluorescence protein live cells yielded five different monoclonal antibodies that were characterized by enzyme-linked immunosorbent assay, sodium dodecyl sulfate–polyacrylamide gel electrophoresis/western blot, flow cytometry, immunohistochemistry, and immunoprecipitation. The different behavior of these antibodies suggests that some of them may react against epitopes masked by different glycosylated protein moieties.
Descripción : 11 pages.
Versión del editor: http://dx.doi.org/10.1111/j.1399-0039.2006.00692.x
URI : http://hdl.handle.net/10261/6337
DOI: 10.1111/j.1399-0039.2006.00692.x
ISSN: 0001-2815
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