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Crystallization and preliminary crystallographic studies of human kallikrein 7, a serine protease of the multigene kallikrein family

AuthorsFernández, Israel S. ; Ständker, Ludger; Forssmann, Wolf Georg; Giménez-Gallego, Guillermo ; Romero, Antonio
Keywordshepatitis B core particles
temperature jump
N-terminal fusion
Issue DateAug-2007
PublisherInternational Union of Crystallography
CitationActa Crystallographica, Section F 63(8):669-672(2007)
AbstractHepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20 000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 Å resolution and data were collected to 99.6% completeness at 8.9 Å. The crystal belongs to space group P212121, with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 Å. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells
Description4 páginas, 6 figuras, 2 tablas -- PAGS nros. 669-672
Publisher version (URL)http://dx.doi.org/10.1107/S1744309107031764
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