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Title: | Crystallization and preliminary crystallographic studies of human kallikrein 7, a serine protease of the multigene kallikrein family |
Authors: | Fernández, Israel S. ![]() ![]() ![]() |
Keywords: | hepatitis B core particles cryoprotectant temperature jump N-terminal fusion |
Issue Date: | Aug-2007 |
Publisher: | International Union of Crystallography |
Citation: | Acta Crystallographica, Section F 63(8):669-672(2007) |
Abstract: | Hepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20 000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 Å resolution and data were collected to 99.6% completeness at 8.9 Å. The crystal belongs to space group P212121, with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 Å. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells |
Description: | 4 páginas, 6 figuras, 2 tablas -- PAGS nros. 669-672 |
Publisher version (URL): | http://dx.doi.org/10.1107/S1744309107031764 |
URI: | http://hdl.handle.net/10261/63366 |
DOI: | 10.1107/S1744309107031764 |
ISSN: | 1744-3091 |
Appears in Collections: | (CIB) Artículos |
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Acta Cristallographica F 63 (8) 669-672 (2007).pdf | 228,99 kB | Adobe PDF | ![]() View/Open |
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