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Title: | The use of recombinant gilthead sea bream (Sparus aurata) growth hormone for radioiodination and standard preparation in radioimmunoassay |
Authors: | Martínez-Barberá, Juan P.; Pendón, Carlos; Martí-Palanca, Hilario; Calduch-Giner, Josep A. ![]() ![]() |
Keywords: | Radioimmunoassay Rabbit antiserum Recombinant growth hormone Gilthead seabream Sparus aurata Growth hormone Teleost Fish |
Issue Date: | Apr-1995 |
Publisher: | Elsevier |
Citation: | Comp. Biochem. Physiol. 110A: 335-340 (1995). |
Abstract: | A gilthead sea bream growth hormone (sbGH) obtained by cloning and expression of sbGH cDNA was used to develop a sensitive and specific radioimmunoassay (RIA). Iodination of recombinant sbGH (rsbGH) was performed by the classical Chloramine-T method. Specific antiserum, raised in rabbits, was added in a final dilution of 1/36,000. The minimum detectable dose was 30 pg, and the midrange of the assay (ED50) was 275 pg. Intra- and inter-assay coefficients of variation (CV) were 3.3 and 5.8% at ED50 levels. Human GH (hGH), ovine GH (oGH), carp gonadotropin (cGtH), chinook salmon gonadotropin (sGtH), ovine prolactin (oPRL) and recombinant tilapia prolactin (rtiPRL) did not show cross-reactivity. Serial dilutions of chinook salmon GH (sGH) and recombinant rainbow trout GH (rtGH) showed a low but significant cross-reactivity. A good parallelism between rsbGH standard and serial dilutions of native sbGH, plasma and pituitary extracts was observed. In addition, when plasma and pituitary samples were analyzed for GH quantification, non-significant differences were observed within this and previous RIA for native sbGH. Therefore, it appears conclusive that our rsbGH can be used successfully as a standard and radioiodinated hormone in GH assays for gilthead sea bream, which is extensively cultured in the Mediterranean area. |
Description: | 6 pages. |
Publisher version (URL): | http://dx.doi.org/10.1016/0300-9629(94)00178-V |
URI: | http://hdl.handle.net/10261/6328 |
DOI: | http://dx.doi.org/10.1016/0300-9629(94)00178-V |
ISSN: | 0300-9629 |
Appears in Collections: | (ICMAN) Artículos (IATS) Artículos |
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