English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/6318
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 13 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Gene silencing by RNAi in mouse Sertoli cells
Autor : González-González, Emilio ; López-Casas, Pedro P. ; Del Mazo, Jesús
Palabras clave : RNA interference
Sertoli cells
Gene silencing
Fecha de publicación : 11-jul-2008
Editor: BioMed Central
Citación : Reproductive Biology and Endocrinology 2008, 6:29
Resumen: [Background] RNA interference (RNAi) is a valuable tool in the investigation of gene function. The purpose of this study was to examine the availability, target cell types and efficiency of RNAi in the mouse seminiferous epithelium.
[Methods] The experimental model was based on transgenic mice expressing EGFP (enhanced green fluorescent protein). RNAi was induced by in vivo transfection of plasmid vectors encoding for short hairpin RNAs (shRNAs) targeting EGFP. shRNAs were transfected in vivo by microinjection into the seminiferous tubules via the rete testis followed by square wave electroporation. As a transfection reporter, expression of red fluorescent protein (HcRed 1) was used. Cell types, the efficiency of both transfections and RNAi were all evaluated.
[Results] Sertoli cells were the main transfected cells. A reduction of about 40% in the level of EGFP protein was detected in cells successfully transfected both in vivo and in vitro. However, the efficiency of in vivo transfection was low.
[Conclusion] In adult seminiferous epithelial cells, in vivo post-transcriptional gene silencing mediated by RNAi via shRNA is efficient in Sertoli cells. Similar levels of RNAi were detected both in vivo and in vitro. This also indicates that Sertoli cells have the necessary silencing machinery to repress the expression of endogenous genes via RNAi.
Descripción : 9 pages, 5 figures.-- PMCID: PMC2483279.
Versión del editor: http://dx.doi.org/10.1186/1477-7827-6-29
URI : http://hdl.handle.net/10261/6318
DOI: 10.1186/1477-7827-6-29
ISBN : 1477-7827
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Gene_silencing.pdf533,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.