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dc.contributor.authorKarashima, Yuji-
dc.contributor.authorPrenen, Jean-
dc.contributor.authorMeseguer, Víctor M.-
dc.contributor.authorOwsianik, Grzegorz-
dc.contributor.authorVoets, Thomas-
dc.contributor.authorNilius, Bernd-
dc.identifierdoi: 10.1007/s00424-008-0493-6-
dc.identifierissn: 0031-6768-
dc.identifiere-issn: 1432-2013-
dc.identifier.citationPflugers Archiv European Journal of Physiology 457(1): 77-89 (2008)-
dc.description.abstractThe transient receptor potential channel of the ankyrin-binding repeat subfamily, TRPA1, is a Ca2+-permeable non-selective cation channel that depolarizes the plasma membrane and causes Ca2+ influx. A typical feature of TRPA1 is its rapid desensitization following activation by agonists such as mustard oil (MO), cinnamaldehyde, and a high intracellular Ca2+ concentration. In whole-cell recordings on Chinese hamster ovary (CHO) cells expressing TRPA1, desensitization was delayed when phosphatidylinositol 4,5-biphosphate (PIP2) was supplemented via the patch pipette, whereas the PIP2 scavenger neomycin accelerated desensitization. Preincubation with the PI-4 kinase inhibitor wortmannin reduced both constitutive TRPA1 channels activity and the response to MO. Run down was also accelerated by high intracellular Mg2+ concentrations, whereas chelating intracellular Mg2+ with 10 mM ethylenedinitrilotetraacetic acid (EDTA) increased the basal channel activity. In inside-out patches, we observed a rapid run down of TRPA1 activity, which could be prevented by application of diC8-PIP2 or 2 mM Mg-ATP but not Na2-ATP to the cytosolic side of the excised patches. In isolated trigeminal ganglion neurons, preincubation with wortmannin resulted in inhibition of endogenous TRPA1 activation by MO. Taken together, our data indicate that PIP2 modulates TRPA1, albeit to a lesser extent than other known PIP 2-dependent TRP channels, and that tools modifying the plasma membrane PIP2 content often have direct effects on this channel. © 2008 Springer-Verlag.-
dc.description.sponsorshipThis work was supported by the Human Frontiers Science Programme (HFSP Research Grant Ref. RGP 32/2004), the Belgian Federal Government, the Flemish Government, the Onderzoeksraad KU Leuven (GOA 99/07, F.W.O. G.0214.99, F.W.O. G. 0136.00; F.W.O. G.0172.03, Interuniversity Poles of Attraction Program, Prime Ministers Office IUAP) and the Excellentiefinanciering (EF/95/010).-
dc.titleModulation of the transient receptor potential channel TRPA1 by phosphatidylinositol 4,5-biphosphate manipulators-
dc.description.versionPeer Reviewed-
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