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Title

Quantitative real-time PCR for detection of Acinetobacter baumannii colonization in the hospital environment

AuthorsMcConnell, Michael J.; Pérez-Ordóñez, A.; Pérez-Romero, Pilar; Valencia, R.; Lepe, José A.; Vázquez-Barba, I.; Pachón, Jerónimo
Issue Date2012
PublisherAmerican Society for Microbiology
CitationJournal of Clinical Microbiology 50(4): 1412-1414 (2012)
AbstractA real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR detected contaminated surfaces in 4 h with high sensitivity (100%) compared to conventional culture. Thirty-eight percent of samples were positive by real-time PCR and negative by bacterial culture (false positives), possibly indicating the widespread presence of bacterial DNA that is not associated with viable bacteria Copyright © 2012, American Society for Microbiology. All Rights Reserved.
URIhttp://hdl.handle.net/10261/63029
DOI10.1128/JCM.06566-11
Identifiersdoi: 10.1128/JCM.06566-11
issn: 0095-1137
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