English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/62914
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Report of the European Myeloma Network on multiparametric flow cytometry in multiple myeloma and related disorders

AuthorsRawstron, Andy C.; Orfao, Alberto ; Mateo, Gema; Pérez-Andrés, Martin; San Miguel, Jesús F.
Issue Date2008
PublisherFerrata Storti Foundation
CitationHaematologica 93(3): 431-438 (2008)
AbstractThe European Myeloma Network (EMN) organized two flow cytometry workshops. The first aimed to identify specific indications for flow cytometry in patients with monoclonal gammopathies, and consensus technical approaches through a questionnaire-based review of current practice in participating laboratories. The second aimed to resolve outstanding technical issues and develop a consensus approach to analysis of plasma cells. The primary clinical applications identified were: differential diagnosis of neoplastic plasma cell disorders from reactive plasmacytosis; identifying risk of progression in patients with MGUS and detecting minimal residual disease. A range of technical recommendations were identified, including: 1) CD38, CD138 and CD45 should all be included in at least one tube for plasma cell identification and enumeration. The primary gate should be based on CD38 vs. CD138 expression; 2) after treatment, clonality assessment is only likely to be informative when combined with immunophenotype to detect abnormal cells. Flow cytometry is suitable for demonstrating a stringent complete remission; 3) for detection of abnormal plasma cells, a minimal panel should include CD19 and CD56. A preferred panel would also include CD20, CD117, CD28 and CD27; 4) discrepancies between the percentage of plasma cells detected by flow cytometry and morphology are primarily related to sample quality and it is, therefore, important to determine that marrow elements are present in follow-up samples, particularly normal plasma cells in MRD negative cases.
DescriptionThis is an open access paper.-- et al.
URIhttp://hdl.handle.net/10261/62914
DOI10.3324/haematol.11080
Identifiersdoi: 10.3324/haematol.11080
issn: 0390-6078
e-issn: 1592-8721
Appears in Collections:(IBMCC) Artículos
Files in This Item:
File Description SizeFormat 
Report of the European.pdf192,29 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.