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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Rozas, José Luis | - |
dc.contributor.author | Gómez-Sánchez, L. | - |
dc.contributor.author | Mircheski, Josif | - |
dc.contributor.author | Linares-Clemente, P. | - |
dc.contributor.author | Nieto-González, J. | - |
dc.contributor.author | Vázquez, M. Eugenio | - |
dc.contributor.author | Luján, Rafael | - |
dc.contributor.author | Fernández-Chacón, Rafael | - |
dc.date.accessioned | 2012-12-13T10:49:06Z | - |
dc.date.available | 2012-12-13T10:49:06Z | - |
dc.date.issued | 2012 | - |
dc.identifier | doi: 10.1016/j.neuron.2012.02.019 | - |
dc.identifier | issn: 0896-6273 | - |
dc.identifier.citation | Neuron 74(1): 151-165 (2012) | - |
dc.identifier.uri | http://hdl.handle.net/10261/62811 | - |
dc.description.abstract | Cysteine string protein-α (CSP-α) is a synaptic vesicle protein that prevents activity-dependent neurodegeneration by poorly understood mechanisms. We have studied the synaptic vesicle cycle at the motor nerve terminals of CSP-α knock-out mice expressing the synaptopHluorin transgene. Mutant nerve terminals fail to sustain prolonged release and the number of vesicles available to be released decreases. Strikingly, the SNARE protein SNAP-25 is dramatically reduced. In addition, endocytosis during the stimulus fails to maintain the size of the recycling synaptic vesicle pool during prolonged stimulation. Upon depolarization, the styryl dye FM 2-10 becomes trapped and poorly releasable. Consistently with the functional results, electron microscopy analysis revealed characteristic features of impaired synaptic vesicle recycling. The unexpected defect in vesicle recycling in CSP-α knock-out mice provides insights into understanding molecular mechanisms of degeneration in motor nerve terminals. Cysteine string protein-α (CSP-α) is a synaptic vesicle protein that prevents activity-dependent neurodegeneration. Rozas et al. have used synaptopHluorin imaging and electrophysiology to find alterations in synaptic vesicle priming and recycling at motor nerve terminals of CSP-α KO mice. © 2012 Elsevier Inc. | - |
dc.description.sponsorship | This work has been supported by Spanish MINECO (Juan de la Cierva and FPU Programs, BES2008-002858, BFU2007-66008, BFU2010-15713, ERA-NET NEURON EUI2009-04084, CTQ2009-14431/BQU, SAF2010-20822-C02), Junta de Andalucía (P06-CVI-02392, P07-CVI-02854), Xunta de Galicia (INCITE09 209 084PR), HFSP (RGP 0045/2002-C and CDA0032/2005-C), Instituto de Salud Carlos III and FEDER. | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.rights | closedAccess | - |
dc.title | Motorneurons Require Cysteine String Protein-α to Maintain the Readily Releasable Vesicular Pool and Synaptic Vesicle Recycling | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1016/j.neuron.2012.02.019 | - |
dc.date.updated | 2012-12-13T10:49:06Z | - |
dc.description.version | Peer Reviewed | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
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