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dc.contributor.authorMartínez-Maqueda, D.-
dc.contributor.authorMiralles, Beatriz-
dc.contributor.authorPascual-Teresa, Sonia de-
dc.contributor.authorReverón, Inés-
dc.contributor.authorMuñoz, Rosario-
dc.contributor.authorRecio, Isidra-
dc.identifierissn: 0021-8561-
dc.identifiere-issn: 1520-5118)-
dc.identifier.citationJournal of Agricultural and Food Chemistry 60(35): 8600-8605 (2012)-
dc.description.abstractIn this study, the hypothesis that food-derived opioid peptides besides β-casomorphin 7 might modulate the production of mucin via a direct action on epithelial goblet cells was investigated in HT29-MTX cells used as a model of human colonic epithelium. Seven milk whey or casein peptides, a human milk peptide, and a wheat gluten-derived peptide with proved or probable ability to bind μ- or δ-opioid receptors were tested on the cell culture. Significantly increased secretion of mucins was found after exposure to six of the assayed peptides, besides the previously described β-casomorphin 7, as measured by an enzyme-linked lectin assay (ELLA). Human β-casomorphin 5 and α-lactorphin were selected to study the expression of mucin 5AC gene (MUC5AC), the HT29-MTX major secreted mucin gene. α-Lactorphin showed increased expression of MUC5AC from 4 to 24 h (up to 1.6-fold over basal level expression), although differences were statistically different only after 24 h of exposure. However, this increased expression of MUC5AC did not reach significance after cell treatment with human β-casomorphin 5. In conclusion, six food-derived peptides have been identifed with described or probable opioid activity that induce mucin secretion in HT29-MTX cells. Concretely, α-lactorphin is able to up-regulate the expression of the major secreted mucin gene encoded by these cells. © 2012 American Chemical Society.-
dc.description.sponsorshipThis work was supported by Projects AGL2008-01713, AGL2011-26643, and Consolider-Ingenio FUN-C-Food CSD 2007-063 from Ministerio de Economía y Competitividad. We are participants in the FA1005COST Action INFOGEST on food digestion. D.M.-M. acknowledges CSIC for a JAE Program fellowship.-
dc.publisherAmerican Chemical Society-
dc.titleFood-derived peptides stimulate mucin secretion and gene expression in intestinal cells-
dc.description.versionPeer Reviewed-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
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