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Title

Structural and functional differences among human surfactant proteins SP-A1, SP-A2 and co-expressed SP-A1/SP-A2: role of supratrimeric oligomerization

AuthorsSánchez-Barbero, Fernando; Rivas, Germán ; Steinhilber, Wolfram; Casals, Cristina
KeywordsAnalytical ultracentrifugation
differential scanning calorimetry
immunosuppression
lipopolysaccharide
oligomerization
surfactant protein A1 (SP-A1)
Issue Date15-Sep-2007
PublisherPortland Press
CitationBiochemical Journal 406(3):479-489(2007)
AbstractSP-A (surfactant protein A) is a membrane-associated SP that helps to maintain the lung in a sterile and non-inflamed state. Unlike SP-As from other mammalian species, human SP-A consists of two functional gene products: SP-A1 and SP-A2. In all the functions examined, recombinant human SP-A1 invariably exhibits lower biological activity than SP-A2. The objective of the present study was to investigate why SP-A2 possesses greater biological activity than SP-A1 and what advantage accrues to having two polypeptide chains instead of one. We analysed structural and functional characteristics of recombinant baculovirus-derived SP-A1, SP-A2 and co-expressed SP-A1/SP-A2 using a wide array of experimental approaches such as analytical ultracentrifugation, DSC (differential scanning calorimetry) and fluorescence. We found that the extent of supratrimeric assembly is much lower in SP-A1 than SP-A2. However, the resistance to proteolysis is greater for SP-A1 than for SP-A2. Co-expressed SP-A1/SP-A2 had greater thermal stability than SP-A1 and SP-A2 and exhibited properties of each protein. On the one hand, SP-A1/SP-A2, like SP-A2, had a higher degree of oligomerization than SP-A1, and consequently had lower Kd for binding to bacterial Re-LPS (rough lipopolysaccharide), higher self-association in the presence of calcium and greater capability to aggregate Re-LPS and phospholipids than SP-A1. On the other hand, SP-A1/SP-A2, like SP-A1, was more resistant to trypsin degradation than SP-A2. Finally, the importance of the supratrimeric assembly for SP-A immunomodulatory function is discussed
Description11 páginas, 10 figuras -- PAGS nros. 479-489
Publisher version (URL)http://dx.doi.org/10.1042/BJ20070275
URIhttp://hdl.handle.net/10261/62364
DOI10.1042/BJ20070275
ISSN0264-6021
E-ISSN1470-8728
Appears in Collections:(CIB) Artículos
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