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Heparin increases prolactin and modifies the effects of FGF-2 upon prolactin accumulation in pituitary primary cultures

AuthorsSpuch, Carlos; Diz-Chaves, Yolanda ; Pérez-Tilve, Diego; Mallo, F.
Issue Date2004
PublisherMacmillan Publishers
CitationEndocrine 24: 131- 136 (2004)
AbstractWe have studied the effects of heparin on prolactin accumulation in the medium from primary pituitary cultures, and whether heparin interferes with the effects of fibroblast growth factor-2 (FGF-2) on PRL regulation in vitro. In the absence of exogenous FGF-2, heparin increased prolactin accumulation in the culture medium in a dose-dependent manner. FGF-2 also increased the prolactin levels of primary cells in a time-and dose-dependent manner. However, low doses of heparin reduced the effects of FGF-2, but higher doses of heparin increased the maximal FGF-2-induced prolactin secretion and ED50. In vivo estrogenization of rats resulted in the abolition of FGF-2 capability to promote prolactin release in vitro. However, heparin restored cell responsiveness to FGF-2. Our results suggest that heparin, when present in the medium, binds FGF-2, therefore reducing its ability to interact with FGF receptors in a dose-dependent manner up to a critical molar concentration, at which heparin itself starts to activate the FGF receptor, and strengthens the activation induced by its proper ligand, FGF-2. Prolactin responses to FGF-2 are blocked by estrogen pretreatment, and it is probable that this introduces lactotroph cells in the proliferative stage. In conclusion, heparin modulates PRL secretion and PRL responses to FGF-2 in vitro. © 2004 by Humana Press Inc. All rights of any nature whatsoever reserved.
Identifiersdoi: 10.1385/ENDO:24:2:131
issn: 0969-711X
Appears in Collections:(IC) Artículos
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