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Título : Transformation of maltose into prebiotic isomaltooligosaccharides by a novel alpha-glucosidase from Xantophyllomyces dendrorhous
Autor : Fernández Arrojo, Lucía ; Marín Alberdi, Dolores; Gómez de Segura, María Aránzazu; Linde, Dolores ; Alcalde Galeote, Miguel ; Gutiérrez Alonso, Patricia; Ghazi, Iraj ; Plou Gasca, Francisco José ; Fernández Lobato, María; Ballesteros Olmo, Antonio
Palabras clave : Prebiotics
Isomaltooligosaccharides
Transglycosilation
Oligosaccharides
Glycosidase
Functional foods
Fecha de publicación : 2007
Editor: Elsevier
Citación : Process Biochemistry Volume 42, Issue 11, November 2007, Pages 1530–1536
Resumen: The transglycosylation activity of a novel alpha-glucosidase from the basidiomycetous yeast Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) was studied using maltose as glucosyl donor. The enzyme synthesized oligosaccharides with alpha-(1-2), alpha-(1-4) and alpha-(1-6) bonds. Using 200 g/l maltose, the yield of oligosaccharides was 53.8 g/l, with prebiotic oligosaccharides containing at least one alpha-(1-6) linkage (panose, 6-O-alpha-glucosyl-maltotriose and 6-O-alpha-isomaltosyl-maltose) being the major products (47.1 g/l). The transglycosylatying yield was 3.6 times higher than the observed with the alpha-glucosidase from Saccharomyces cerevisiae (53.8 vs. 14.7 g/l). Moreover, when increasing the maltose concentration up to 525 g/l, the maximum production of tri- and tetrasaccharides reached 167.1 g/l, without altering the percentage of oligosaccharides in the mixture. Compared with other microbial alpha-glucosidases in which the main transglycosylation product is a disaccharide, the enzyme from X. dendrorhous yields a final product enriched in trisaccharides and tetrasaccharides.
Versión del editor: http://dx.doi.org/10.1016/j.procbio.2007.08.007
URI : http://hdl.handle.net/10261/6225
DOI: 10.1016/j.procbio.2007.08.007
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