English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/62151
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Downregulation of protein tyrosine phosphatase PTPL1 alters cell cycle and upregulates invasion-related genes in prostate cancer cells

AuthorsCastilla, Carolina; Flores, M. Luz; Conde, José M.; Medina López, Rafael Antonio; Torrubia, Francisco J.; Japón, Miguel A. ; Sáez, Carmen
Issue Date2012
PublisherKluwer Academic Publishers
CitationClinical and Experimental Metastasis 29(4): 349-358 (2012)
AbstractPTPL1, a non-receptor type protein tyrosine phosphatase, has been involved in the regulation of apoptosis and invasiveness of various tumour cell types, but its role in prostate cancer remained to be investigated. We report here that downregulation of PTPL1 by small interfering RNA in PC3 cells decreases cell proliferation and concomitantly reduces the expression of cell cycle-related proteins such as cyclins E and B1, PCNA, PTTG1 and phospho-histone H3. PTPL1 downregulation also increases the invasion ability of PC3 cells through Matrigel coated membranes. cDNA array of PTPL1-silenced PC3 cells versus control cells showed an upregulation of invasion-related genes such as uPA, uPAR, tPA, PAI-1, integrin α6 and osteopontin. This increased expression was also confirmed in PTPL1-silenced DU145 prostate cancer cells by quantitative real time PCR and western blot. These findings suggest that PTPL1 is an important mediator of central cellular processes such as proliferation and invasion. © 2012 Springer Science+Business Media B.V.
DescriptionThe final publication is available at link.springer.com
Publisher version (URL)http://dx.doi.org/10.1007/s10585-012-9455-7
URIhttp://hdl.handle.net/10261/62151
DOI10.1007/s10585-012-9455-7
Identifiersdoi: 10.1007/s10585-012-9455-7
issn: 0262-0898
Appears in Collections:(IBIS) Artículos
Files in This Item:
File Description SizeFormat 
downregulation_ protein_tyrosine_castilla.pdf959,33 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.