English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/61992
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Isolation and characterisation of symbiotically effective Rhizobium resistant to arsenic and heavy metals after the toxic spill at the Aznalcóllar pyrite mine

AuthorsCarrasco, J. A.; Armario, P.; Pajuelo, Eloísa; Burgos, Araceli; Caviedes, M. A.; López Núñez, Rafael ; Chamber, Manuel; Palomares, A. J.
Issue Date2005
PublisherPergamon Press
CitationSoil Biology and Biochemistry 37: 1131- 1140 (2005)
AbstractAfter the toxic spill occurred at Aznalcóllar pyrite mine (Southern Spain), a wide area of croplands near the Doñana Wild Park was contaminated with 4.5 million m3 of slurries composed of acidic waters loaded with toxic metals and metalloids such as As, Sb, Zn, Pb, Cu, Co, Tl, Bi, Cd, Ag, Hg and Se. Today, 6 years after the spill, the concentration of toxic elements in these soils is still very high, in spite of the efforts to clean the zone. However, some plant species have colonised this contaminated area. Legumes possessing N2-fixing nodules on their roots represented a significant proportion of these plants. Our objective was to use the Rhizobium-legume symbiosis as a new tool for bioremediate the affected area. We have isolated about 100 Rhizobium strains, 41 of them being resistant to high concentrations of As (300 mg l-1), Cu (100 mg l-1) and Pb (500 mg l-1). Their phenotypes and bioaccumulation potentials have been characterised by their growth rates in media supplemented with As and heavy metals. The presence of the resistance genes in some strains has been confirmed by PCR and Southern blot hybridisation. Several Rhizobium were symbiotically effective in the contaminated soils. On the other hand, the first steps in nodule establishment seemed to be more affected by heavy metals than N 2-fixation.
URIhttp://hdl.handle.net/10261/61992
DOI10.1016/j.soilbio.2004.11.015
Identifiersdoi: 10.1016/j.soilbio.2004.11.015
issn: 0038-0717
Appears in Collections:(IRNAS) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.