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Título : Immobilization of Pycnoporus coccineus laccase on Eupergit C: stabilization and treatment of olive oil mill wastewaters
Autor : Berrio, Jimmy; Plou Gasca, Francisco José ; Ballesteros Olmo, Antonio ; Martínez, Ángel T. ; Martínez, María Jesús
Palabras clave : Olive oil wastewaters
Immobilized enzymes
Phenoloxidases
Epoxy supports
Enzyme bioremediation
Polyacrylamide
Fecha de publicación : 2007
Editor: Informa Healthcare
Citación : Biocatalysis and Biotransformation, Volume 25, Issue 2 - 4 March 2007 , pages 130 - 134
Resumen: The use of olive oil mill wastewaters (OMW) as organic fertilizer is limited by its phytotoxic effect, due to the high concentration of phenolic compounds. As an alternative to physico-chemical methods for OMW detoxification, the laccase from Pycnoporus coccineus, a white-rot fungus that is able to decrease the chemical oxygen demand (COD) and colour of the industrial effluent, is being studied. In this work, the P. coccineus laccase was immobilized on two acrylic epoxy-activated resins, Eupergit C and Eupergit C 250L. The highest activity was obtained with the macroporous Eupergit C 250L, reaching 110 U g-1 biocatalyst. A substantial stabilization effect against pH and temperature was obtained upon immobilization. The soluble enzyme maintained ≥80% of its initial activity after 24 h at pH 7.0-10.0, whereas the immobilized laccase kept the activity in the pH range 3.0-10.0. The free enzyme was quickly inactivated at temperatures above 50 oC, whereas the immobilized enzyme was very stable up to 70 oC. Gel filtration profiles of the OMW treated with the immobilized enzyme (for 8 h at room temperature) showed both degradation and polymerization of the phenolic compounds.
Versión del editor: http://dx.doi.org/10.1080/10242420701379122
URI : http://hdl.handle.net/10261/6194
DOI: 10.1080/10242420701379122
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