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Title

Proteomic and transcriptomic analyses of differential stress/inflammatory responses in mandibular lymph nodes and oropharyngeal tonsils of European wild boars naturally infected with Mycobacterium bovis

AuthorsNaranjo, María Victoria ; Villar, Margarita ; Martín-Hernando, Mari Paz ; Vidal, Dolors ; Höfle, Ursula ; Gortázar, Christian ; Kocan, Katherine M.; Vázquez, Jesús ; Fuente, José de la
Issue Date2007
PublisherJohn Wiley & Sons
CitationProteomics 7: 220- 231 (2007)
AbstractDifferential stress/inflammatory responses were characterized at the mRNA and protein levels in mandibular lymph nodes (MLN) and oropharyngeal tonsils of European wild boars (Sus scrofa), naturally infected with Mycobacterium bovis. Suppression-subtractive hybridization combined with immunohistochemistry and/or quantitative real-time RT-PCR were used to identify and characterize abundant stress/inflammatory gene sequences differentially expressed in tuberculous (TB+) wild boars. Genes identified in MLN and tonsils corresponded to serum amyloid A, arginase I, osteopontin, lysozyme, annexin I, and heat shock proteins, respectively. Global protein patterns in MLN and tonsils were compared between TB+ and nontuberculous (TB-) boars by 2-DE and MALDI-TOF MS. Five proteins, including stress/inflammatory proteins annexin V, serum albumin, and apolipoprotein A1 were found at lower levels in MLN of TB+ boars. Manganese superoxide dismutase was found up-regulated in MLN of TB+ boars. Five proteins, including creatine kinase and MHC class II antigens were found up-regulated in tonsils of TB+ boars. These results demonstrated differential stress/inflammatory responses in wild boars naturally infected with M. bovis and suggest possible markers of tuberculosis in this species that may prove useful for future studies of host-pathogen interactions and for diagnostics and vaccine development. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
URIhttp://hdl.handle.net/10261/61374
DOI10.1002/pmic.200600527
Identifiersdoi: 10.1002/pmic.200600527
issn: 1615-9853
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