English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/61288
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Mesenchymal stem cells from multiple myeloma patients display distinct genomic profile as compared with those from normal donorsGenomic profile of multiple myeloma MSCs

AuthorsGarayoa, Mercedes ; García, Juan L. ; Santamaría, Carlos; García-Gómez, Antonio; Blanco, Juan F.; Pandiella, Atanasio ; Hernández, Jesús M. ; Sánchez-Guijo, Fermín M.; Cañizo, María Consuelo del; Gutiérrez, Norma Carmen; San Miguel, Jesús F.
Issue Date2009
PublisherNature Publishing Group
CitationLeukemia 23: 1515-1527 (2009)
AbstractIt is an open question whether in multiple myeloma (MM) bone marrow stromal cells contain genomic alterations, which may contribute to the pathogenesis of the disease. We conducted an array-based comparative genomic hybridization (array-CGH) analysis to compare the extent of unbalanced genomic alterations in mesenchymal stem cells from 21 myeloma patients (MM-MSCs) and 12 normal donors (ND-MSCs) after in vitro culture expansion. Whereas ND-MSCs were devoid of genomic imbalances, several non-recurrent chromosomal gains and losses (>1Mb size) were detected in MM-MSCs. Using real-time reverse transcription PCR, we found correlative deregulated expression for five genes encoded in regions for which genomic imbalances were detected using array-CGH. In addition, only MM-MSCs showed a specific pattern of 'hot-spot' regions with discrete (<1Mb) genomic alterations, some of which were confirmed using fluorescence in situ hybridization (FISH). Within MM-MSC samples, unsupervised cluster analysis did not correlate with particular clinicobiological features of MM patients. We also explored whether cytogenetic abnormalities present in myelomatous plasma cells (PCs) were shared by matching MSCs from the same patients using FISH. All MM-MSCs were cytogenetically normal for the tested genomic alterations. Therefore we cannot support a common progenitor for myeloma PCs and MSCs.
URIhttp://hdl.handle.net/10261/61288
DOI10.1038/leu.2009.65
Identifiersdoi: 10.1038/leu.2009.65
issn: 0887-6924
e-issn: 1476-5551
Appears in Collections:(IBMCC) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.