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Title

Macroporous scaffolds based on chitosan and bioactive molecules

AuthorsKirsebom, Harald; Aguilar, María Rosa ; San Román, Julio ; Fernández, Margarita ; Prieto, María Auxiliadora ; Bondar, Borys
KeywordsChitosan
Cytocompatibility
RGD
Cryogels
Scaffold
Issue DateNov-2007
PublisherSage Publications
CitationJournal of Bioactive and Compatible Polymers 22(6):621-636(2012)
AbstractChitosan-based macroporous scaffolds for tissue engineering applications are developed by cryogelation in aqueous media. The cryogels obtained are modified using a new RGD-containing peptide developed in this laboratory. A RGD-containing peptide is chemically attached to the surface of the cryogels to improve cell adhesion to the 3D-structure chitosan-based scaffolds. The synthesis, physico-chemical, and biological evaluations of the system are described, and the optimization of the formulations is carried out by varying the reaction parameters. Fibroblasts and endothelial cells are used in cell cultures to determine cell behavior and the cytocompatibility of the macroporous cryogels. Cell spreading and actin cytoskeleton organization process are assessed by confocal microscopy. Cells colonize the porous structure of the chitosan-based cryogel and are observed to be growing inside the pores.
Publisher version (URL)http:dx.doi.org/10.1177/0883911507084293
URIhttp://hdl.handle.net/10261/61250
DOI10.1177/0883911507084293
ISSN0883-9115
E-ISSN1530-8030
Appears in Collections:(CIB) Artículos
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