English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/61249
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

DC FieldValueLanguage
dc.contributor.authorNavarro-Villa, A.-
dc.contributor.authorO'Brien, M.-
dc.contributor.authorLópez, Secundino-
dc.contributor.authorBoland, T. M.-
dc.contributor.authorO'Kiely, P.-
dc.identifier.citationAnimal Feed Science and Technology (166-67 Special Issue) : 163-174 (2011)es_ES
dc.description12 páginas, 4 figuras, 6 tablas.es_ES
dc.description.abstractThe objective was to modify an in vitro rumen gas production technique (GPT) so that CH(4) production from feeds with contrasting compositions would better reflect in vivo findings. A phosphate-bicarbonate saline solution was mixed with citric acid and used as the buffer. Varying quantities (i.e., 0.3, 0.5, 0.7 g) of each of three contrasting feeds (i.e., barley grain. grass silage, barley straw) were incubated with 50 ml of varying ratios of rumen fluid to buffer (1:2, 1:4, 1:6). Effects on CH(4) output/g feed dry matter (DM) incubated (CH(4)/DMi) and/g DM disappeared (CH(4)/DMd) and other associated changes were evaluated after 24 h of incubation. The in sacco degradability of barley straw, grass silage and barley grain DM was 212, 682 and 888 g/kg, respectively. Increasing the quantity of feed incubated in vitro led to a decline in CH(4)/DMi and CH(4)/DMd and a decline in pH (P<0.001). Increasing the proportion of rumen fluid in the mixture increased CH(4)/DMi and CH(4)/DMd and reduced pH (P<0.01). Results were consistent among feeds tested. However for grass silage, apparent DM disappearance declined (P<0.001) as the quantity of feed incubated increased, an outcome associated with a simultaneous decline in pH. CH(4)/DMd is a more appropriate unit for expressing in vitro rumen CH(4) output than CH(4)/DMi and incubation of 0.3 g dried milled feed with 50 ml of rumen fluid and buffer mixture at a 1:2 ratio was a satisfactory combination with this buffer system.es_ES
dc.description.sponsorshipNational Development Plan through the Research Stimulus Fund (RSF 06361)es_ES
dc.subjectIn vitroes_ES
dc.subjectRumen fluides_ES
dc.titleModifications of a gas production technique for assessing in vitro rumen methane production from feedstuffses_ES
dc.description.peerreviewedPeer reviewedes_ES
Appears in Collections:(IGM) Artículos
Files in This Item:
There are no files associated with this item.
Show simple item record

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.