English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/60945
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Auto-regulation of the synthesis of the MobM relaxase encoded by the promiscuous plasmid pMV158

AuthorsLorenzo-Díaz, Fabián ; Solano-Collado, Virtu ; Lurz, Rudi; Bravo, Alicia ; Espinosa, Manuel
Issue DateApr-2012
PublisherAmerican Society for Microbiology
CitationJournal of Bacteriology 194(7):1789-1799(2012)
AbstractThe streptococcal promiscuous plasmid pMV158 (5,540 bp) replicates by the rolling-circle mechanism and can be mobilized among a wide number of Gram-positive and -negative bacteria. The plasmid region involved in its conjugative transfer includes the mobM gene, which encodes the MobM relaxase, and the cis-acting origin of transfer (oriT). MobM initiates transfer by cleavage of supercoiled pMV158 DNA at a specific dinucleotide within oriT. In the present work, we have performed a detailed transcriptional analysis to assess the role of MobM in the control of its own gene expression. By in vivo and in vitro approaches, we demonstrated that mobM transcription in Escherichia coli was mostly initiated from a promoter (Pmob2) different from the one (Pmob1) used in Lactococcus lactis. Whereas promoter Pmob1 was embedded within the oriT sequence, promoter Pmob2 was placed apart from but adjacent to oriT. Further, MobM was able to repress the expression of its own gene from both promoters. Given the promiscuity of pMV158, the organization of the mobM promoter region suggests a strategy of the plasmid to cope with different transcription machineries of the hosts it colonizes
Description11 páginas, 9 figuras, 1 tabla -- PAGS nros. 1789-1799
Publisher version (URL)http://jb.asm.org/content/194/7/1789
URIhttp://hdl.handle.net/10261/60945
DOI10.1128/JB.06827-11
ISSN0021-9193
E-ISSN1098-5530
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
J. Bacteriology-2012-revised version.pdf1,68 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.