English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/60926
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

A HPLC method for specific determination ofα-amylase and glucoamylase in complex enzymatic preparations

AuthorsFranco, José M. ; Murado García, Miguel Anxo ; Siso, M. I. G. ; Mirón, Jesús ; González Fernández, Pilar
KeywordsColumn liquid chromatography
Glucose, maltose and maltotriose
α-Amylase and glucoamylase
Microfungal cultures
Issue Date1989
PublisherSpringer
CitationChromatographia 27(7-8): 328-332 (1989)
AbstractIn the hydrolysis of soluble starch by mixtures of α-amylase and glucoamylase, the ratios maltose/glucose and maltoriose/glucose linearly depend, over a wide range, on the relation between both enzymes and are independent on the activity level of the enzymatic preparation. HPLC determination of hydrolysis products (glucose, maltose and maltotriose) of soluble starch by mixtures of these enzymes, after incubation under controlled conditions, is a rapid method for the evaluation of the relative levels of each enzyme in the mixtures. The method, first developed using pure commercial amylases, is applied, with consistent results, to cell free media ofAspergillus niger cultures on a glycogen-rich effluent.
Description5 páginas, 4 figuras, 4 tablas
Publisher version (URL)http://dx.doi.org/10.1007/BF02321279
URIhttp://hdl.handle.net/10261/60926
DOI10.1007/BF02321279
ISSN0009-5893
E-ISSN1612-1112
Appears in Collections:(IIM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.