Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/60926
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A HPLC method for specific determination ofα-amylase and glucoamylase in complex enzymatic preparations |
Autor: | Franco, José M. CSIC; Murado García, Miguel Anxo; Siso, María I. G. CSIC; Mirón, Jesús CSIC ORCID; González Fernández, Pilar CSIC | Palabras clave: | Column liquid chromatography Glucose, maltose and maltotriose α-Amylase and glucoamylase Microfungal cultures |
Fecha de publicación: | 1989 | Editor: | Springer Nature | Citación: | Chromatographia 27(7-8): 328-332 (1989) | Resumen: | In the hydrolysis of soluble starch by mixtures of α-amylase and glucoamylase, the ratios maltose/glucose and maltoriose/glucose linearly depend, over a wide range, on the relation between both enzymes and are independent on the activity level of the enzymatic preparation. HPLC determination of hydrolysis products (glucose, maltose and maltotriose) of soluble starch by mixtures of these enzymes, after incubation under controlled conditions, is a rapid method for the evaluation of the relative levels of each enzyme in the mixtures. The method, first developed using pure commercial amylases, is applied, with consistent results, to cell free media ofAspergillus niger cultures on a glycogen-rich effluent. | Descripción: | 5 páginas, 4 figuras, 4 tablas | Versión del editor: | http://dx.doi.org/10.1007/BF02321279 | URI: | http://hdl.handle.net/10261/60926 | DOI: | 10.1007/BF02321279 | ISSN: | 0009-5893 | E-ISSN: | 1612-1112 |
Aparece en las colecciones: | (IIM) Artículos |
Mostrar el registro completo
CORE Recommender
SCOPUSTM
Citations
9
checked on 20-abr-2024
WEB OF SCIENCETM
Citations
9
checked on 26-feb-2024
Page view(s)
372
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.