English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/60510
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Human initiator caspases trigger apoptotic and autophagic phenotypes in Saccharomyces cerevisiae

AuthorsLisa-Santamaría, Patricia ; Neiman, Aaron M.; Cuesta-Marbán, Álvaro ; Mollinedo, Faustino ; Revuelta Doval, José Luis ; Jiménez, Alberto
Issue Date2009
CitationBBA - Molecular Cell Research1793(3): 561-571 (2009)
AbstractCaspases are a family of proteases that participate in the progression and execution of the apoptotic program. However, regulation of the caspase activation and their substrates has not yet been fully elucidated. Here we explore the effect of the ectopic expression of the human initiator caspases-8 and -10 in Saccharomyces cerevisiae. Our results showed that the expression of human CASP10 and CASP8 triggers certain apoptotic markers such as a massive production of reactive oxygen species (ROS), chromatin condensation and phosphatidylserine externalization, finally leading to cell death. In response to hydroxyurea (HU), yeast cells expressing caspase-10 did not reduce the replication of DNA and escaped to the intra-S checkpoint of the cell cycle. In addition, caspase-10 expression induced yeast vacuolization and a vacuole-associated phenotype resembling autophagy. Other intracellular alterations such as disorganization of the actin cytoskeleton, cell wall damage, and aberrations within the endoplasmic reticulum lumen were also associated with caspase-10 expression. Furthermore, caspase-induced cell death was completely dependent on the proteolytic activation of the enzyme but, in contrast, was not dependent on either of the endogenous yeast apoptotic proteins Aif1 and Mca1 or the mitochondria. © 2008 Elsevier B.V.
Identifiersdoi: 10.1016/j.bbamcr.2008.12.016
issn: 0167-4889
Appears in Collections:(IBMCC) Artículos
(IMB) Artículos
Files in This Item:
File Description SizeFormat 
Human initiator caspases.pdf2,97 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.