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Separation of paralytic shellfish toxins by reversed phase high performance liquid chromatography, with postcolumn reaction and fluorimetric detection

AuthorsFranco, José M. ; Fernández-Vila, Pablo
KeywordsColumn liquid chromatography
Ion pairing
Paralytic shellfish toxins
Dinoflagellate cultures
Toxic molluscs
Issue Date1993
CitationChromatographia 35(9-12): 613-620 (1993)
AbstractAn RP-HPLC method for the separation of shellfish toxins is described, employing three isocratic systems: Octanesulfonic acid in ammonium phosphate buffer plus acetonitrile for the separation of carbamate toxins (neoSTX, dcSTX and STX), octansulfonic acid in ammonium phosphate buffer for the separation of GTXs and tetrabutylammonium in the same buffer for Cs separation. These eluents perform the separation prior to postcolumn reaction and fluorimetric detection. The procedures have been applied to the determination of toxin production in dinoflagellate cultures, field samples of red tides, and toxic mussels.
Description8 páginas, 4 figuras, 1 tabla
Publisher version (URL)http://dx.doi.org/10.1007/BF02267925
Appears in Collections:(IIM) Artículos
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