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Characterization of a 65 kDa NIF in the nuclear matrix of the monocot Allium cepa that interacts with nuclear spectrin-like proteins

AuthorsPérez Munive, C.; Blumenthal, S.; Moreno Díaz de la Espina, Susana
KeywordsAllium cepa
lamin-like protein
nuclear intermediate filament
Issue Date5-Sep-2012
CitationCell Biology International, 36 : 1097-1105 (2012)
AbstractPlant cells have a well organized nucleus and nuclear matrix, but lack orthologs of the main structural components of the metazoan nuclear matrix. Although data is limited, currently, the main plant nuclear structural proteins are coiled-coil proteins such as the NIFs reported in Pisum sativum that cross-react with anti-intermediate filament and anti-lamin antibodies, form filaments 6-12 nm in diameter in vitro and have been proposed to functionally play the role of lamins. The aim of this study was to investigate the conservation and features of NIFs in a monocot species Allium cepa and compare them with the previously described onion lamin-like proteins. For that we used polyclonal antisera against the pea 65 kDa NIF in 1D and 2D western blots, ICM and IEM. Their presence in the nuclear matrix was analyzed by differential extraction of nuclei, and their association with structural spectrin-like proteins by co-immunoprecipitation and co-localization in ICM. We demonstrated that the 65 kDa NIF is a conserved structural component of the nucleus and nuclear matrix in monocots with Mr and pI values similar to those of the pea 65 kDa NIF that localized to the nuclear envelope, perichromatin domains and foci and to the nuclear matrix, and interacted directly with structural nuclear spectrin-like proteins. Its similarities with some of the proteins previously described as onion lamin-like proteins suggest that they could be highly related or even the same proteins
Description9 páginas, 5 figuras -- PAGS nros. 1105-1097
Publisher version (URL)http://dx.doi.org/10.1042/CBI20120237
Appears in Collections:(CIB) Artículos
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