English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/60043
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

DC FieldValueLanguage
dc.contributor.authorBarderas, Rodrigo-
dc.contributor.authorDesmet, Johan-
dc.contributor.authorAlard, P.-
dc.contributor.authorCasal, J. Ignacio-
dc.date.issued2012-10-01-
dc.identifier.citationMethods in Molecular Biology, 907 : 463-86 (2012)es_ES
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10261/60043-
dc.description24 páginas, 3 figuras -- PAGS nros. 463-486es_ES
dc.description.abstractRational engineering methods can be applied with success to optimize physicochemical characteristics of antibodies. Application of in silico analysis and prediction methods to antibody Fv regions can help to find residues affecting antibody–antigen affinity when high-resolution antibody structures or antibody–antigen complex structures are known. In these cases, the identification of residues affecting affinity can facilitate the selection of candidates for guided maturation by PCR using degenerate oligonucleotides. Here, we describe the utilization of a semi-rational approach to enhance the affinity of antibodies by combining in silico and traditional wet lab-based methodses_ES
dc.description.sponsorshipThis work was supported by a grant from the Spanish Ministry of Science and Innovation BIO2009-08818es_ES
dc.language.isoenges_ES
dc.publisherHumana Presses_ES
dc.rightsclosedAccesses_ES
dc.subjectmaturationes_ES
dc.subjectsilico modelinges_ES
dc.subjectantibody engineeringes_ES
dc.subjectphage displayes_ES
dc.titleAffinity maturation by semi-rational approacheses_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1007/978-1-61779-974-7_27-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1007/978-1-61779-974-7_27es_ES
dc.identifier.e-issn1940-6029-
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
restringido.pdf21,67 kBAdobe PDFThumbnail
View/Open
Show simple item record
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.