English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/59461
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

HER2 carboxyl-terminal fragments regulate cell migration and cortactin phosphorylation

AuthorsGarcía-Castillo, Jesús; Pedersen, Kim; Angelini, Pier-Davide; Bech-Serra, Joan Josep; Colomé, Nuria; Cunningham, Matthew Paul; Parra-Palau, Josep Lluís; Canals, Francesc; Baselga, José; Arribas, Joaquín
Issue Date2009
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry 284(37): 25302- 25313 (2009)
AbstractA group of breast cancer patients with a higher probability of developing metastasis expresses a series of carboxyl-terminal fragments (CTFs) of the tyrosine kinase receptor HER2. One of these fragments, 611-CTF, is a hyperactive form of HER2 that constitutively establishes homodimers maintained by disulfide bonds, making it an excellent model to study overactivation of HER2 during tumor progression and metastasis. Here we show that expression of 611-CTF increases cell motility in a variety of assays. Since cell motility is frequently regulated by phosphorylation/dephosphorylation, we looked for phosphoproteins mediating the effect of 611-CTF using two alternative proteomic approaches, stable isotope labeling with amino acids in cell culture and difference gel electrophoresis, and found that the latter is particularly well suited to detect changes in multiphosphorylated proteins. The difference gel electrophoresis screening identified cortactin, a cytoskeleton-binding protein involved in the regulation of cell migration, as a phosphoprotein probably regulated by 611-CTF. This result was validated by characterizing cortactin in cells expressing this HER2 fragment. Finally, we showed that the knockdown of cortactin impairs 611-CTF-induced cell migration. These results suggest that cortactin is a target of 611-CTF involved in the regulation of cell migration and, thus, in the metastatic behavior of breast tumors expressing this CTF.
URIhttp://hdl.handle.net/10261/59461
DOI10.1074/jbc.M109.001982
Identifiersdoi: 10.1074/jbc.M109.001982
issn: 0021-9258
e-issn: 1083-351X
Appears in Collections:(IN) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.