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Título

cDNA-AFLP analysis of seed germination in Arabidopsis thaliana identifies transposons and new genomic sequences

AutorGutiérrez de Diego, Juana; Rodríguez García, David; Rodríguez Lorenzo, José Luis; Grappin, Philippe; Cervantes, Emilio
Palabras claveArabidopsis
cDNA-AFLP
Germination
Gibberellic acid
Transposon
Fecha de publicaciónmar-2006
EditorElsevier
CitaciónJournal of Plant Physiology 163(4): 452-462 (2006)
ResumenA cDNA-AFLP experiment was designed to identify and clone nucleotide sequences induced during seed germination in Arabidopsis thaliana. Sequences corresponding to known genes involved in processes important for germination, such as mitochondrial biogenesis, protein synthesis and cell cycle progression, were isolated. Other sequences correspond to Arabidopsis BAC clones in regions where genes have not been annotated. Notably, a number of the sequences cloned did not correspond to available sequences in the databases from the Arabidopsis genome, but instead present significant similarity with DNA from other organisms, for example fish species; among them, some may encode transposons. A number of the sequences isolated showed no significant similarity with any sequences in the public databases. Oligonucleotides derived from these new sequences were used to amplify genomic DNA of Arabidopsis. Expression analysis of representative sequences is presented. This work suggests that, during germination, there may be a massive transposon mobilization that may be useful in the annotation of new genome sequences and identification of regulatory mechanisms.
Descripción11 pages, 4 figures.-- PMID: 16455359 [PubMed].-- Available online Aug 3, 2005.
Versión del editorhttp://dx.doi.org/10.1016/j.jplph.2005.04.032
URIhttp://hdl.handle.net/10261/5944
DOI10.1016/j.jplph.2005.04.032
ISSN0176-1617
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