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In vitro studies on the regulation of rainbow trout (Oncorhynchus mykiss) macrophage respiratory burst activity

AuthorsNovoa, Beatriz ; Figueras Huerta, Antonio ; Ashton, Ian; Secombes, Christopher J.
Rainbow trout
Respiratory burst
Issue Date1996
PublisherPergamon Press
CitationDevelopmental and Comparative Immunology 20(3): 207-216 (1996)
AbstractModulation of the respiratory burst activity of head kidney macrophages isolated from rainbow trout (Oncorhynclrus mykiss) was observed following treatment with several biologically active substances. Macrophage-activating factor (MAF) induced the highest increment in respiratory burst activity relative to treatment with lipopolysaccharide (LPS), tumor necrosis factor a (TNFa) or pglucans from Saccharomyces cerevisiac. Increased responses were more evident when these molecules were combined in pairs. Negative regulation of respiratory burst activity was observed when diMePG& was added to the macrophages, with maximal inhibition seen using a concentration of 2.6 W. Inhibition was also seen using stimulated macrophages, either by co-incubation of stimuli and diMePGEz or by adding di- MePGEs to previously stimulated cells. The inhibitory effect on macrophages was detectable within 3 h of incubation with diMePG& and by 24 h the level of the response was even lower than that from unstimulated (control) macrophages. Of significance was the finding that the inhibitory effect of prostaglandin on macrophage function could be overcome by co-incubation with stimulatory molecules or by pre-treatment with MAF and LPS or MAF and TNFu. Thus, the regulation of macrophage activation in fish is likely to be as complex as in mammals.
Description10 páginas, 4 figuras
Publisher version (URL)http://dx.doi.org/10.1016/0145-305X(96)00011-0
Appears in Collections:(IIM) Artículos
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