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Reversed-phase HPLC as a method for the identification of gadoid fish species

AuthorsPiñeiro, Carmen ; González Sotelo, Carmen ; Medina, Isabel ; Gallardo, José Manuel ; Pérez Martín, Ricardo Isaac
KeywordsFish species
Sarcoplasmic proteins
Issue Date1997
CitationZeitschrift für Lebensmitteluntersuchung und -Forschung A 204(6): 411-416 (1997)
AbstractA simple and rapid method to identify raw or frozen gadoid fish species is described. The methodology is based on the HPLC separation of water-soluble proteins and monitoring at 230 nm. Chromatographic profiles were reproducible between individuals belonging to the same species; lower variations in the relative retention time than in peak area percentage were observed. Results obtained from an ANOVA test allowed the distinction of characteristic peaks. Profiles were independent of fish quality: fish refrigerated for 10 days showed the same HPLC protein profile as those that were fresh. The ability of the identification procedure was checked by analysis of a set of frozen commercial samples.
Description6 páginas, 5 tablas, 6 figuras.-- The original publication is available at www.springerlink.com
Publisher version (URL)http://dx.doi.org/10.1007/s002170050103
Appears in Collections:(IIM) Artículos
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