English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/59052
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


A new protein carrying an NmrA-like domain is required for cell differentiation and development in Dictyostelium discoideum

AuthorsNúñez-Corcuera, Beatriz ; Serafimidis, Ioannis; Arias-Palomo, Ernesto ; Rivera-Calzada, Angel ; Suárez, Teresa
Prestalk A cells
Ammonium transporters
Pattern formation
Issue Date15-Sep-2008
CitationDevelopmental Biology 321(2):331-342(2008)
AbstractWe have isolated a Dictyostelium mutant unable to induce expression of the prestalk-specific marker ecmB in monolayer assays. The disrupted gene, padA, leads to a range of phenotypic defects in growth and development. We show that padA is essential for growth, and we have generated a thermosensitive mutant allele, padA−. At the permissive temperature, mutant cells grow poorly; they remain longer at the slug stage during development and are defective in terminal differentiation. At the restrictive temperature, growth is completely blocked, while development is permanently arrested prior to culmination. padA− slugs are deficient in prestalk A cell differentiation and present an abnormal ecmB expression pattern. Sequence comparisons and predicted three-dimensional structure analyses show that PadA carries an NmrA-like domain. NmrA is a negative transcriptional regulator involved in nitrogen metabolite repression in Aspergillus nidulans. PadA predicted structure shows a NAD(P)+-binding domain, which we demonstrate that is essential for function. We show that padA− development is more sensitive to ammonia than wild-type cells and two ammonium transporters, amtA and amtC, appear derepressed during padA− development. Our data suggest that PadA belongs to a new family of NAD(P)+-binding proteins that link metabolic changes to gene expression and is required for growth and normal developmen
Description12 páginas, 10 figuras, 1 tabla, 3 figuras suplementarias -- PAGS nros. 331-342
Publisher version (URL)http://dx.doi.org/10.1016/j.ydbio.2008.06.027
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
restringido.pdf21,67 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.