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Title

Isolation and characterization of microsatellite loci in the Cape fynbos heath Erica coccinea (Ericaceae)

AuthorsSegarra-Moragues, José G. ; Donat-Caerols, S. ; Ojeda Copete, Fernando
KeywordsCape floristic region
Genetic diversity
Resprouter
Seeder
SSR
Issue DateDec-2009
PublisherSpringer
CitationConservation Genetics (10)6: 1815-1819 (2009)
AbstractEnriched genomic libraries were used to isolate and characterize dinucleotide microsatellite loci in Erica coccinea, a South African Cape fynbos heath species with distinct resprouter and seeder populations. Microsatellites were required to investigate the effect of the contrasting demographic pattern driven by these two post-fire responses in the population genetic structure of seeder and resprouter forms within this species. Eight microsatellite loci were characterised and amplified a total of 106 alleles in 2 samples each of 30 individuals from 1 resprouter and 1 seeder population. Mean allele numbers were 7.88 and 11.0 for the resprouter and seeder population, respectively. Both populations showed similar average observed and expected heterozygosity levels, H (O)(resprouter) = 0.683, H (O)(seeder) = 0.696; H (E)(resprouter) = 0.726, H (E)(seeder) = 0.756, and average positive inbreeding coefficients F (IS)(resprouter) = 0.058, F (IS)(seeder) = 0.080. This set of microsatellite loci will be used to conduct a population genetic survey of seeder and resprouter populations throughout the range of the species. Cross-species transferability was also assayed in four other South African and four European species of the genus Erica, supporting their potential use for population genetic analyses.
Description5 paginas, 2 tablas.
Publisher version (URL)http://dx.doi.org/10.1007/s10592-009-9823-2
URIhttp://hdl.handle.net/10261/58965
DOI10.1007/s10592-009-9823-2
ISSN1566-0621
E-ISSN1572-9737
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