English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/58882
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


In vitro interaction of viral haemorrhagic septicaemia virus and leukocytes from trout (Oncorhynchus mykiss) and turbot (Scophthalmus maximus)

AuthorsTafalla, Carolina; Figueras Huerta, Antonio ; Novoa, Beatriz
Fish rhabdovirus
Respiratory burst
Issue Date1998
CitationVeterinary Immunology and Immunopathology 62(4): 359-366 (1998)
AbstractViral haemorrhagic septicaemia virus VHSV., a well known salmonids pathogen, has also been reported to be pathogenic for turbot Scophthalmus maximus.. In the present work, the replication of VHSV was studied in vitro in turbot head kidney macrophages and blood leukocytes. VHSV was able to infect both primary cultures and viral titer increased with time, either inside the cells or in the supernatant. However, no cytopathic effect was observed during the experiments and the titers were always lower than those obtained in the fish cell lines. The number of trout and turbot macrophages after several days of in vitro infection with VHSV was compared with uninfected controls by viable cell count but no significant differences were observed. The number of cells supporting viral replication evaluated by immunofluorescence in trout and turbot was low 8 and 1.7%, respectively.. Respiratory burst activity of head kidney macrophages was assayed at different days post-infection, but no significant differences were found between the control and the infected cultures neither in trout nor turbot
Description8 páginas, 2 figuras
Publisher version (URL)http://dx.doi.org/10.1016/S0165-2427(97)00167-0
Appears in Collections:(IIM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.